FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColTGA5_Col_UB_f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColTGA5_Col_UB_f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913815
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGACC1550.007376007713.54859937
CCACTGT3551.592184E-510.8449577
ACTGTAG4552.2028053E-69.9998969
TCCACTG4652.9083621E-69.7848436
CTAGTGG3803.7585414E-49.2104310
CACTGTA4855.0624683E-58.6597038
CTATTGC4159.050326E-48.4332071
CTATTGG7654.200756E-77.77729031
CTGTAGA6602.912539E-57.42416510
CGCAACC13003.569403E-86.192404346
CTATTGA11701.3506906E-65.9825311
GAGTATA27750.05.92802128
AAACCGC19859.094947E-125.81857942
GATTGAG25000.05.7402424
GCGGTTT17857.530616E-105.68636365
CCGCAAC16101.4080797E-85.652262745
GCAACCC7450.00677685955.63752516
TGTCATG20753.274181E-115.566206512
GTCATGT20351.1459633E-105.503628313
TGCGGTT19107.1122486E-105.497324564
Produced by FastQC (version 0.11.5)