FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColTGA5_Col_UB_f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColTGA5_Col_UB_f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967150
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3050.036.7199136
CCACTGT5000.034.998677
TCCACTG6000.031.49886
ACTGTAG7600.031.3153929
GGACTGT3950.027.4673087
CACTGTA6500.025.8451678
CGGACTA3150.024.4435126
CTGTAGA9550.023.82168610
CCGGACT2400.023.3324435
GCGGACT1652.6666385E-923.3324435
TCGGACT2700.020.7399485
GACTGTA4800.020.4158888
GCCGGAC1107.4816286E-419.0901814
GGACTAG4150.018.5535097
CCACTAG5300.018.489867
CTAGTGG4950.018.38360610
GTAGAAG13350.018.08965912
CGGACTC1953.9548286E-717.9480346
CAGTCCG2551.1295924E-917.84336527
ATCCACT9450.017.0363885
Produced by FastQC (version 0.11.5)