FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColTGA5_Col_B_f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColTGA5_Col_B_f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491067
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6149212.52212019948398No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC7560.15395047926250388Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5150.1048736730425787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT6700.065.2991760
CGCCCTA650.064.6160459
CGTATCA6850.063.86926362
CGCCGTA6100.063.68917559
CCGTATC6500.063.53911261
TCGCCGT5850.061.0262658
GGTCGCC7450.060.12871656
TGTACCG352.9722287E-760.0006118
GTCGCCG7250.058.8911657
CGTGCAT308.372468E-658.3339345
TGGTCGC6600.057.79773355
CGTCACG553.6379788E-1157.2674853
GGTCCCC800.056.86978556
TGGACCG252.3895636E-456.0005725
GCGCCAC252.3895636E-456.000578
TAGCCGT1750.056.0005758
AGCCGTA1900.055.26371859
CCCTATC1150.054.78316561
CGTGCCA452.6991984E-854.44499610
CCACTCG452.6991984E-854.44499614
Produced by FastQC (version 0.11.5)