FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColTGA5_Col_B_f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColTGA5_Col_B_f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915947
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG838159.150638628654278No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC29930.3267656316358916Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGG252.8406503E-669.99950444
GCCGTAT14500.067.1029860
CGCCGTA13050.065.9765559
TCGCCGT12300.065.7312558
GGTCGCC16350.065.7181956
CCGTATC13050.065.7083561
GTCGCCG16050.064.5478457
TGGTCGC15050.063.7204855
CGTATCA14500.061.55129262
GTGGTCG16600.061.35498454
GGTGGTC16950.060.91402453
GCGCCGT1700.059.70546358
TCGGTGG14100.059.5740551
CGGTGGT15650.058.59383852
CTCGGTG10450.056.6024750
CCCGTGC252.3908628E-455.9996073
CCTGGCC252.3908628E-455.99960760
GCCCGGC252.3908628E-455.99960710
GGTCCCC1700.055.58784556
GTCCCCG1450.055.51685357
Produced by FastQC (version 0.11.5)