FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColTGA5_Col_B_f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColTGA5_Col_B_f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608510
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG486878.001018882187639No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC10050.1651575158994922Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCACA150.002224058170.0027135
CCCCGTT150.002225508469.99126
GCCGTAT8450.066.2747660
CGCCCTA1750.064.0024859
CCGTATC7900.063.7999461
GGTCGCC9450.063.70617356
TCGCCCT1650.063.6388358
CGCCGTA7550.063.5123959
CCCCGTA1000.063.0024459
GCGCCGT1350.062.22463658
CCGTGGT850.061.76709752
CCGTATA2100.061.6690661
GTCGCCG8600.061.04887857
TGGTCGC8400.060.83569355
CGTATCA8750.060.4023462
GCCCTAT2300.059.35012460
GGTAGCC2300.059.35012456
TCGCCGT7400.058.65092558
GGTCCCC1450.057.93327756
GGTGGTC9750.057.7971153
Produced by FastQC (version 0.11.5)