FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColSPL9_pool19011A_input_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColSPL9_pool19011A_input_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4109593
Sequences flagged as poor quality0
Sequence length76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG391390.9523814158725694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATT143750.067.0488054
GTAAATT202050.066.911515
CGTAATT65300.066.61824
TAATTAC367950.066.4112856
GTAATTA411900.066.315665
TAAATTA698000.066.0862056
CTAATTA199950.065.933975
CTAAATT138000.065.860935
TAATTAT398650.065.774396
CCGTAAT21950.065.690563
AGTAATT132250.065.443044
CATAATT108250.065.306814
CCTAATT52950.065.235834
GCTAATT54750.065.2005164
GATAATT124200.064.9507754
CTTAATT76000.064.883224
TAATTAG247450.064.608476
GTTAATT106750.064.519684
CCATAAT34500.064.4161763
CGGTAAT33450.064.345663
Produced by FastQC (version 0.11.5)