FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColSPL9_pool19011A_UB_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColSPL9_pool19011A_UB_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9594932
Sequences flagged as poor quality0
Sequence length76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1081031.126667703325047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGT982100.068.526178
CCACTAG1093250.068.493337
ATCCACT1279300.068.320935
CCACTCT624700.068.205167
TCCACTA1217150.068.1634146
CTCCACT793000.068.086935
ACTAGTG977950.068.016739
TCCACTC709550.067.950966
TCCACTG1572400.067.511726
CTAGTGG986400.067.2956810
AATCCAC520700.067.140624
TATCCAC371250.067.112514
GTCCACT657900.067.0348365
CCACTGT1363100.066.862937
TTCCACT915000.066.842325
ATTCCAC362150.066.7597664
CCTCCAC231600.066.4464
ACTCCAC302550.066.3764954
GCTCCAC168550.066.342694
CATCCAC304800.066.127654
Produced by FastQC (version 0.11.5)