FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColSPL9_pool19011A_B_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColSPL9_pool19011A_B_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4588124
Sequences flagged as poor quality0
Sequence length76
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160850.35057901660896695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAT28250.061.9443365
GGCGCTA20300.061.0312964
CGGTAAT58400.060.8865623
GGGCGCT9200.060.8657233
GGCGCTC12350.060.64464
GGGTAAT59250.058.8317223
GGTAATC63400.058.73514
CCGCTAG14250.057.9625135
GGTAATA112700.057.854124
GCGCTAG15500.057.8040585
CGCTATT30700.057.457536
CCGCTAT24750.057.4117625
GTAATCT99100.057.389155
GCGGCGC11450.057.1598322
GTAATAT171750.056.8739175
GCGTAAT29000.056.841233
AGGTAAT84450.056.2368243
GTAATAG82950.056.0314375
CGCTAGT18700.055.399386
TGGTAAT90300.055.0740363
Produced by FastQC (version 0.11.5)