FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColSPL9_SPL9_F1b_B_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColSPL9_SPL9_F1b_B_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16900
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1370.8106508875739644No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCCAC453.6379788E-1270.000014
TATATCC150.002187062570.02
CGCCCTG150.002187062570.065
AAATTCA150.002187062570.057
ACCACTG150.002187062570.06
ACGTCCA150.002187062570.03
TGTCCAC207.705123E-570.04
GGTCCAC207.705123E-570.04
TTATCCA252.7216793E-670.03
TTTTCCA150.002187062570.03
CTCCACT1150.070.05
ACTCTTC700.070.09
GTATCCA150.002187062570.03
ACTCTCT207.705123E-570.09
TACATCC150.002187062570.02
ACATCCA353.426976E-970.03
ACTTCCA150.002187062570.03
CCACCTG150.002187062570.07
CCACTGG150.002187062570.07
CCACTCT1100.070.07
Produced by FastQC (version 0.11.5)