FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColLHY_F1a_B_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColLHY_F1a_B_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241990
Sequences flagged as poor quality0
Sequence length76
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2960.12231910409521055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCGA200.00694519352.50046567
TCTCCTA700.00122369325.00022129
TAGCATA850.003782052820.58841762
TATAGTC1250.001757066316.80014868
Produced by FastQC (version 0.11.5)