FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColCAMTA1_F1a_B_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColCAMTA1_F1a_B_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622265
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14940.24009063662587482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT800.002664914621.87623260
TAGGGTG1859.948053E-920.81030838
GTGGGAC850.003789369520.58773847
GGTCGCC1301.0561897E-418.84721456
GAGTCGG1000.00968239217.50098257
TGGTCGC1000.00968687217.49957855
TCAGACG1554.0521612E-415.8060710
CGGTGGT1859.0255904E-515.1347752
AGGGTGT2604.851354E-714.80733639
GATGCTC1450.004734894714.48357341
TGTGCTC1550.00737059813.5480617
TTTGGGC1550.00737059813.5480626
ACGCTTC1550.00737059813.5480636
GTGGTCG1550.00737059813.5480654
GGGTGTA2354.556851E-513.4050140
GGTGGTC1600.0090913813.12468453
ATAGGGT2405.5567514E-513.12468337
GATAGGG2254.7443737E-412.44414436
GTGTAGA3100.00682865979.03276642
AGAGTGT4703.48933E-58.93595527
Produced by FastQC (version 0.11.5)