FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColCAMTA1_Col_B_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColCAMTA1_Col_B_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549061
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA501.709335E-434.99995458
GCCGCTT500.00720718627.99996442
TCGTCCC800.002665409821.8749715
CAACCCT1000.00968418917.49997748
ATTGTGC1000.00968418917.49997768
TCTGATC2408.1578677E-411.66665164
CTCATAC2550.001354913110.98037758
AGCGTCG2903.2389082E-410.86205570
ATCTTGG2350.00924219710.42551870
TACTTAG3750.00340142058.39998945
AGAGCGT3750.00340142058.39998970
ATCGGAA7951.0264557E-88.36476970
GATCGGA8101.43245416E-88.20986669
CTTGTCT4350.00143578638.0466991
GCTACAC4000.00608167337.8749945
AAAGGTT5159.0697914E-47.47571942
AAGAGCG4700.0030743747.44679969
TAGATGA4750.00340942257.36841154
CTATATA8704.1194107E-77.2420291
ATTATCA6753.0604785E-46.7413461
Produced by FastQC (version 0.11.5)