FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_ColCAMTA1_C24_B_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_ColCAMTA1_C24_B_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866879
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30520.35206758959439555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTG3104.7948277E-511.29029570
GGACAAT2500.001148914611.2006181
CGGAAGG3208.0331246E-49.84372570
TCGGATA2900.0039472989.65514869
CGGAAGC8202.0918378E-108.96339370
TCGTGTT7702.9823925E-56.818165370
GTCGTGG8904.55629E-66.68537667
AGCGTCG27150.06.31674370
GTCGTGT18851.8189894E-126.12730669
TCGTGGA7050.00366622475.957432368
GAGCGTC28200.05.957432369
CGTGGAG7200.00463445255.833318769
CGTGTAG13252.0524112E-65.54715670
AGAGCGT28750.05.478247669
CGTCGTG25201.8189894E-125.27776568
TAGGGAA9450.00297365665.18517267
TGTAGGG11652.7753323E-45.107283668
Produced by FastQC (version 0.11.5)