FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_C24CAMTA1_F1b_B-f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_C24CAMTA1_F1b_B-f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339632
Sequences flagged as poor quality0
Sequence length76
%GC35

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10140.2985584397229943No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTCC150.002223551370.02
CCACTAG11000.065.863647
TCCACTC7300.065.2054756
TCCACTG19250.064.5454566
CCACTGT16700.064.131747
CTAGTGG9500.064.1052610
CCACTCT6350.063.9370087
ACTAGTG9800.063.9285749
TCCACTA12950.063.7837876
CTCCACT9450.063.70375
ATCCACT14250.063.1228035
CACTAGT10600.062.4056558
CACTGTA16000.062.1249968
GTCCACT8500.061.352945
CCACTGG1150.060.8695647
ACTGTAG16300.059.26389
GCTCCAC1950.059.230774
CGTCCAC2250.059.111114
ACTCCAC4000.058.6249964
TTCCACT10900.058.440375
Produced by FastQC (version 0.11.5)