FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_C24CAMTA1_F1a_B-f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_C24CAMTA1_F1a_B-f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534235
Sequences flagged as poor quality0
Sequence length76
%GC34

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10590.198227371849467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACTG11850.062.9124036
CCACTAG6700.062.1651767
CACTAGT6100.061.394438
CCACTGT10650.061.1277437
CTAGTGG5950.059.4127210
ACTGTAG9700.059.176219
CACTGTA10400.056.875918
ATCCACT11350.056.7356875
TCCACTA8900.056.630126
GTCCACT4450.056.624825
ACTAGTG6600.056.2130249
CTCCACT6050.055.5328835
TCCACTC5650.054.5141536
CTGTAGA10250.053.26914610
CCACTCT5650.052.0362367
TTCCACT8050.051.300375
TAGTGGA7700.047.7280411
TATCCAC4450.044.8279844
AATCCAC6350.044.642214
GCTCCAC1900.044.2070964
Produced by FastQC (version 0.11.5)