FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n02_C24CAMTA1_C24_B-f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n02_C24CAMTA1_C24_B-f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147598
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8350.5657258228431279No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCG207.90577E-570.000011
AATCCGC207.90577E-570.000012
TCCGCTC2100.070.000014
CCCTCGC455.456968E-1270.000012
ACCGCTG401.3460522E-1070.000014
CGACCGC650.070.000012
ACTTCGC401.3460522E-1070.000012
CCTACGC455.456968E-1270.000012
GCTGCCG207.90577E-570.000011
GCGCCAG207.90577E-570.000015
CGCTGTA1300.070.000016
CTGCGCT1300.070.000013
ATCCGCT900.070.000013
CAGGGCG207.90577E-570.000011
CCACTGC207.90577E-570.000017
TGCGCTT401.3460522E-1070.000014
TGCGCTG900.070.000014
CCGTAAC207.90577E-570.0000170
AGGCCGC455.456968E-1270.000012
ACCTACG207.90577E-570.000011
Produced by FastQC (version 0.11.5)