FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18164602
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238820.13147549282940524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG660800.065.915997
CCACTGT1120550.065.6643147
TCCACTG1210000.065.201326
CTAGTGG672900.064.4710410
CACTAGT644200.064.120738
CACTGTA1078900.064.020568
ACTAGTG645950.063.9849369
TCCACTA717300.063.7156686
ACTGTAG1134850.063.639919
TTCCACT588050.063.305325
ATCCACT709800.063.2882585
GTCCACT415500.061.409565
CTGTAGA1147950.060.5984510
CCACTCT264050.058.9317367
TAGTGGA729950.058.8375711
CGGACTG99700.058.3983426
CCTCCAC245500.058.013054
TGTAGAA1052800.057.5950111
CGTCCAC123900.057.446344
GCTTGAA1003000.057.40681516
Produced by FastQC (version 0.11.5)