FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_Ler_Lib10_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_Ler_Lib10_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345672
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTC150.002223584370.000011
CTTGTCC301.0221629E-770.000012
CTGCTGT150.002223584370.0000147
CATGGTC150.002223584370.000011
TTGATAT150.002223584370.0000114
AGCGTCC301.0221629E-770.000012
CTGTCTT150.002223584370.0000110
GTACCTC455.456968E-1270.000011
TCGCATC301.0221629E-770.000011
TCCATGG150.002223584370.000016
GCCTTCC600.070.000012
GTACCAC455.456968E-1270.000014
AATCATC455.456968E-1270.000011
ACGCCCA150.002223584370.0000117
AGTTTCC1200.070.000012
TCTCTGG150.002223584370.0000164
CTATCTT150.002223584370.0000110
ACTTGTC150.002223584370.000011
ACCATCC1550.070.000012
CCGGCTC150.002223584370.000011
Produced by FastQC (version 0.11.5)