FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_Col_Lib09_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_Col_Lib09_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654516
Sequences flagged as poor quality0
Sequence length76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG750.070.010021
TACCGCG750.070.010021
ATTTACG2700.070.010011
AACCGCG353.7052814E-970.010011
AGCCGCG301.0225813E-770.010011
TACCCCG850.070.010011
CAAACCG1000.070.010011
TGCCCCG500.070.010011
TTGGACG301.0225813E-770.010011
TACTTCG550.070.010011
CTCCGCG650.070.010011
CAACGCG800.070.010011
ACTTGCG600.070.010011
CCTAGCG500.070.010011
ACGCACG455.456968E-1270.010011
TCCTGCG700.070.010011
ATGTGCG600.070.010011
ATAGCCG650.070.010011
AGGACCG650.070.010011
CCCAGCG500.070.010011
Produced by FastQC (version 0.11.5)