FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_ColTGA5_Col_UB_f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_ColTGA5_Col_UB_f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916525
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGGAAGAGCACACGTCTGAACTCCAGTCACTAA10980.11980033277870217TruSeq Adapter, Index 3 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGT9690.10572543029377267TruSeq Adapter, Index 3 (97% over 36bp)
CGCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGTG9660.10539810698016966TruSeq Adapter, Index 3 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGG9640.10517989143776765TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAG16850.064.391691
CGTCGGA16500.061.9393921
CGCGGAA29450.061.799661
CGTAGGA6000.060.6666681
CAAGCGG5150.059.8058241
CGTACGG5250.058.6666681
GGCGACC4200.056.666679
TGATACG7600.055.263163
GTCGGAA18050.055.0692562
GATACGG7400.054.8648644
GTACGGA5450.053.9449542
GTAGGAA6650.053.6842082
AAGCGGA5750.052.3478242
TACGGCG4750.051.5789456
CGTGGAA9950.050.6532671
GTGGAAG10200.049.75492
ATACGGC5150.048.2524265
GCGACCA4500.048.2222210
CGGCGAC4950.048.0808078
CGTAGCC1550.047.4193531
Produced by FastQC (version 0.11.5)