FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_ColTGA5_Col_UB_f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_ColTGA5_Col_UB_f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913815
Sequences flagged as poor quality0
Sequence length76
%GC34

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG900.005278133319.4444376
TGCCTTG5350.016.3551354
GTGCGTG1350.002947570515.5555550
CGCAGTG1450.00474144114.48275510
GAGCCCC1500.005937153513.99999546
ATGGGCC2100.0040087611.66666321
GCCTTGA5209.484211E-910.7692275
ATGCGTC2650.001870810610.5660333
TATCGCC2350.009256219510.42552866
CTATTCG4055.997892E-610.3706371
TATGCGT4451.6569593E-610.2247152
ATGCCTT7503.6379788E-119.7999963
CGTCTCC2900.0039509199.65516956
GCAACCC6553.3778633E-99.61831847
CTAGTGG4158.8617744E-59.27710410
TATGCCT7554.2018655E-109.271522
TCGAGCA3500.00182386578.99999751
CCGCAAC15650.08.945683545
CGCAACC11400.08.90350546
CCACTGT3550.00207492788.8732377
Produced by FastQC (version 0.11.5)