FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_ColTGA5_Col_UB_f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_ColTGA5_Col_UB_f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967150
Sequences flagged as poor quality0
Sequence length76
%GC34

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG2250.043.5555236
CCACTGT5600.034.9999737
TCCACTG6700.030.8208736
ACTGTAG8700.028.1608989
CGGACTA2950.027.2881156
CTGTAGA10500.025.66664910
CACTGTA6850.025.0364788
GGACTGT3950.023.9240347
CTAGTGG5150.023.7863910
TAGTCCG2950.023.72879611
GCGGACT2108.367351E-1121.666655
CTAGTCC3500.019.99998710
TCGGACT3000.019.8333195
TGCGGAC900.00527819519.444434
CCGGACT2551.1295924E-917.8431225
GTCCACT4600.017.4999895
CCACTAG5450.017.3394367
GTAGAAG14550.017.31957412
GACTGTA4450.017.3033588
ACTAGTG6700.017.2387949
Produced by FastQC (version 0.11.5)