FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_ColTGA5_Col_B_f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_ColTGA5_Col_B_f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491067
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGGAAGAGCACACGTCTGAACTCCAGTCACTAA6920.14091763445721256TruSeq Adapter, Index 3 (96% over 33bp)
CGCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGTG5920.12055381444894485TruSeq Adapter, Index 3 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCC353.7071004E-970.01
CGCGGAA21750.063.5632171
CGGGAAG12200.063.4016381
GGCGACC2650.062.0754749
CGTACGG3400.061.7647061
GTACGGA3450.059.8550762
CGTTCGG1000.059.5000041
CGTCGGA12300.058.902441
TGATACG4350.058.7356343
CAAGCGG4150.057.34941
TACGGCG3400.056.6176456
GATACGG4150.056.5060274
CGTTGCC1950.055.6410261
CGCGGCA1450.055.5172421
CGTAGGA5550.055.49551
CGGCGAC3100.055.322588
GTCGGAA12350.054.6963542
ATACGGC3200.054.6875045
CGTATCG1350.054.4444431
GACGTTC452.6993803E-854.4444433
Produced by FastQC (version 0.11.5)