FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_ColTGA5_Col_B_f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_ColTGA5_Col_B_f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915947
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGGAAGAGCACACGTCTGAACTCCAGTCACTAA11400.12446134983792731TruSeq Adapter, Index 3 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGT9950.10863073955152426TruSeq Adapter, Index 3 (97% over 36bp)
CGCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGTG9300.10153425907830911TruSeq Adapter, Index 3 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAG16850.062.314541
CGCGGAA30700.061.7915341
CGTACGG5200.061.251
CGTCGGA17900.060.027931
CAAGCGG5300.058.7735861
CGTAGGA6500.054.3846171
AAGCGGA5750.054.1739162
GTCGGAA19600.053.035712
ACTCGGA3500.052.019
GTACGGA5950.051.764712
GATACGG6950.051.3669054
CGCGGCA2800.051.251
TGATACG7450.050.7382553
CGTTGCC2900.050.689661
ATACGGC5000.049.0000045
GGCGACC4850.048.3505139
TACTCGG3700.048.24324418
TACGGCG5250.048.06
ACGGCGA5150.046.8932087
GCGACCA4650.046.66666810
Produced by FastQC (version 0.11.5)