FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_ColTGA5_Col_B_f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_ColTGA5_Col_B_f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608510
Sequences flagged as poor quality0
Sequence length76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAG10300.061.5048521
CAAGCGG3450.060.8695681
CGCGGAA17350.059.5100861
CGTACGG3800.058.0263141
CGTAGGA4900.057.1428571
CGTCGGA11500.056.6086921
GGCGACC2050.056.3414659
GTAGGAA5050.054.0594062
GATACGG4100.053.7804874
GTCGGAA11800.053.389832
TGATACG4400.053.2954523
CGCGTAC200.006950473452.57
CACTGTA1550.051.9354828
TACGGCG2450.051.4285746
CCACTGT1750.050.07
ATACGGC2450.048.571435
GTACGGA4550.047.6923072
CGGCGAC2450.047.1428578
CGCTACG305.87721E-446.66666410
CGTATGC7300.046.0273971
Produced by FastQC (version 0.11.5)