FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_ColSPL9_pool19011A_input_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_ColSPL9_pool19011A_input_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4109593
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGCT31650.068.452423
GGTAATT218650.068.3358464
GCGGCGC19300.068.005782
TAATTTA648200.067.991966
AGGGCGC8600.067.965712
CGTAATT93100.067.7825554
GTAATTT229900.067.7169955
CGGTAAT47400.067.711573
GCTAATT101700.067.694794
GGGTAAT90350.067.676313
GATAATT174200.067.549394
GTAATTA372100.067.470365
AGTAATT178750.067.1810154
GCGCCGC19500.067.12882
CAGCGCT24500.067.0005953
GCCGCTA71150.066.9998864
CGCCGCT41250.066.946053
TAATTAC402800.066.9426
GTTAATT177300.066.921074
CCTAATT100900.066.878694
Produced by FastQC (version 0.11.5)