FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_ColSPL9_pool19011A_UB_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_ColSPL9_pool19011A_UB_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9594932
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG1166350.068.346467
CACTAGT1094500.068.0876168
TCCACTA1245000.067.942436
ACTAGTG1090000.067.7233059
ATCCACT1149800.067.394595
TCCACTG1820700.066.891846
CTAGTGG1124350.066.85898610
TTCCACT953150.066.8569955
CCACTGT1669550.066.798757
CCACTCT442600.066.053917
CACTGTA1578350.065.99518
TATCCAC359650.065.961614
GTCCACT653300.065.6928945
CATCCAC293900.065.5225454
ACTGTAG1547550.065.3658149
TGTCCAC200500.065.252124
CCTCCAC317350.065.191684
CGTCCAC174750.065.13334
CTTCCAC253850.065.1194154
ATTCCAC340450.065.117014
Produced by FastQC (version 0.11.5)