FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_ColSPL9_pool19011A_B_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_ColSPL9_pool19011A_B_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4588124
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCT31300.065.6393663
CCGCTAG49100.065.366985
CGCTAGA85350.065.284516
GGGCGCT30450.065.057863
GCGCTAG42350.065.041715
GGGTAAT102100.064.858373
GCCGCTA55150.063.5271224
CGCCGCT31200.062.8208853
GGCGCGC13800.062.64532
GCGCTAT33200.061.9883235
CGGCGCT28800.061.858013
GAGCGCT33050.061.740153
GCCCGCT17000.061.5591853
GGCCGCT20900.061.292233
CGGTAAT58900.061.2057953
GGCGCTA49800.061.004384
GCGTAAT49200.060.894673
GGCTAAT55400.060.144763
GGGGTAA33650.060.1192252
GGATAAT119050.059.769363
Produced by FastQC (version 0.11.5)