FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_ColSPL9_SPL9_F1b_B_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_ColSPL9_SPL9_F1b_B_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16900
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG1450.070.000017
CTAGTGG1800.070.0000110
TCTCCAC453.6379788E-1270.000014
TCCTCCA150.002187062570.03
TATATCC207.705123E-570.02
TTTCCAC401.2187229E-1070.04
TGTCCAC207.705123E-570.04
GTTCCAC252.7216793E-670.04
GGTCCAC252.7216793E-670.04
TAGTCCA207.705123E-570.03
ACTTCCA207.705123E-570.03
CCACTAT207.705123E-570.07
CCTCCAC353.426976E-970.04
ATAGTCC150.002187062570.02
TATCCAC750.070.04
CACTCTA150.002187062570.08
TTCCACT1600.070.05
GTCTCCA150.002187062570.03
CCTTCCA150.002187062570.03
TCCACTG2650.070.06
Produced by FastQC (version 0.11.5)