FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_ColCAMTA1_F1a_B_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_ColCAMTA1_F1a_B_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622265
Sequences flagged as poor quality0
Sequence length76
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT8190.131615951403341TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3800.038.6842148
GTATGCC3750.038.26666647
CGCTCAT8000.036.75000434
ATGCCGT3650.036.4383649
CCGCTCA8300.036.2650633
CACCGCT8900.034.2134831
GCCGTCT2200.033.40909251
ACCGCTC8600.033.37209332
GTCACCG9550.033.35078429
TCACCGC9350.033.3155130
AGTCACC9600.032.812528
CGTATGC4300.032.5581446
TGCCGTC3700.032.16216350
CTCGTAT4000.031.50000244
CGCGCGC450.004306957631.11111313
GCTCATT8950.030.89385635
TCTCGTA4300.030.1162843
CCGTCGT1551.8189894E-1229.35484152
CCAGTCA10850.029.3548426
CAGTCAC10350.029.08212727
Produced by FastQC (version 0.11.5)