FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_ColCAMTA1_Col_B_f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_ColCAMTA1_Col_B_f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549061
Sequences flagged as poor quality0
Sequence length76
%GC33

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGC400.00241736435.002879
CTCCGCT1003.9302636E-420.999811
AACACCC1250.001761119616.79984759
TCCGCTT1300.002288889816.15372
CATCGTT1550.00736841613.54826467
TATGCCT1850.001546533313.2431222
GGTTGCG2550.001355000910.98029261
CGTCTGA2300.00788040510.65207769
GATAGAG2350.00924273110.42543740
ACGCGCT2350.00924273110.42543761
GTCATGT3803.744194E-49.21128213
TGTCATG4006.2495086E-48.75071712
GTTGCGG3650.00266367788.63005862
CGGAAGA7453.033165E-88.45629969
ATATTCG5957.270668E-68.2352191
TCATGTG3850.00430980058.18248914
AGAGATC4300.0012820918.13946167
GAGATCG3900.0048447088.0768568
GAGTATA4804.2712243E-48.02076128
TGCGGTT3950.0054326057.974611364
Produced by FastQC (version 0.11.5)