FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_C24CAMTA1_F1b_B-f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_C24CAMTA1_F1b_B-f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339632
Sequences flagged as poor quality0
Sequence length76
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG10800.065.786767
CTAGTGG10500.065.3330610
ACTAGTG10500.064.333069
CCACTGT17250.064.318577
TCCACTA12400.063.2255366
CACTGTA16900.062.9583138
TCCACTG18750.062.7197386
CACTAGT10950.061.6892368
GTCCACT7000.061.4997445
ATCCACT11350.061.365385
ACTGTAG17550.060.2276659
CCACTCT5150.058.4463587
CTGTAGA17250.058.43453610
CGTCCAC2100.058.3330884
AGTCCAC2950.058.135354
TAGTGGA11800.058.1353511
CTCCACT10800.056.0645755
CCGTCCA750.055.9997673
TTCCACT12000.055.9997675
AATCCAC4800.053.2289434