FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_C24CAMTA1_F1a_B-f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_C24CAMTA1_F1a_B-f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534235
Sequences flagged as poor quality0
Sequence length76
%GC35

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTGT12300.062.0319377
CACTGTA11150.061.524098
TCCACTG13800.060.8689966
ACTGTAG10800.060.606969
CCACTAG7000.059.9994437
CTAGTGG6750.057.56040210
CACTAGT6950.056.4023488
ACTAGTG6950.055.9039889
GTCCACT4650.055.6984065
CGTCCAC1800.054.443934
CTGTAGA10900.054.27062610
ATCCACT8700.053.907545
TCCACTA8850.052.20296
CTCCACT8900.047.9770775
TTCCACT8550.047.8942875
TAGTGGA7800.047.5681111
GCTCCAC2650.046.2259834
CCACTCT3400.045.2936947
CCTCCAC3250.045.2303434
GATCCAC2800.044.9995774
Produced by FastQC (version 0.11.5)