FastQCFastQC Report
Thu 21 Feb 2019
HFWYWAFXY_n01_C24CAMTA1_C24_B-f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYWAFXY_n01_C24CAMTA1_C24_B-f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147598
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGC1150.070.000012
TGCCCCG207.90577E-570.000011
GGGTCGC1150.070.000012
ACCGCCG207.90577E-570.000011
GCGCCGC1600.070.000012
GCGCCCG401.3460522E-1070.000011
CCGCTGA1150.070.000015
CGCTGAC401.3460522E-1070.000016
GCCATCG207.90577E-570.000011
CCTCGCT650.070.000013
GAGACGC207.90577E-570.000012
AGGCCCG207.90577E-570.000011
GAGCGCG401.3460522E-1070.000011
CGCTACC1050.070.000016
TTGACGC401.3460522E-1070.000012
TGTTCGC800.070.000012
TCGTCGC650.070.000012
CGAATAG207.90577E-570.000018
GTTCCCG207.90577E-570.000011
CGGTGCG207.90577E-570.000011
Produced by FastQC (version 0.11.5)