FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14605947
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG870030.5956683260592415No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN304880.20873689326683167No Hit
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC170210.11653472383543496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGCA29000.038.24849714
TGCGTGG45550.037.46589710
GGGCAAA32300.034.79518516
GGGGCAA33450.033.59592415
CGCTAGG15550.033.2377846
TGCCTAG14900.031.30088266
ATGTCAT64050.031.185041
GTGATCG16150.031.17381752
GCGTGGG50600.030.61793311
CGTGGGG50450.030.44427112
GGCAAAG37000.030.37693217
GCTAGGT17300.029.87516847
CGGTGGA17950.029.82415433
GCCTAGT16350.028.73566467
GAGAGAG19100.028.71481138
TAGTCTC15650.028.00598570
GGTGATC17850.027.61242951
GGAGAGA20900.027.07888437
CTAGTCT18450.025.08512569
TCAACTG23750.024.8331743
Produced by FastQC (version 0.11.5)