FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n02_Sorb_9_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n02_Sorb_9_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5825579
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC139430.23934101657534812No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT138300.23740129521889583No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT119480.2050954935123187No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT103170.17709827641166656No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC102870.17658330614004203No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA96930.16638689476187687No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG93750.16092820988265716No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC91700.1574092463598897No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT90420.15521203986762516No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT88470.1518647331020659No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA82680.141925806859713No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA81530.1399517541518191No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC81290.13953977793451947No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC79120.13581482630310224No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC76230.1308539460197862No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA71550.12282040978244393No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA69750.11973058815269694No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG67280.11549066624965518No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT65700.11277848948576615No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA64660.1109932592108012No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT64120.11006631272187709No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC62550.10737130163370887No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT62370.10706231947073415No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTAT62290.10692499406496762No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT60980.10467629054554063No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT59590.10229026162034709No Hit
GATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTC59440.10203277648453485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA4750.042.73586715
CGCTAGG5850.042.47766546
TGGGGCA18350.041.57943314
GCTAGGT6250.039.1991147
GGGCAAA20200.038.4644716
GTGATCG6050.037.60244852
TTTGCGA5700.036.8444258
GGAGAGA6850.036.78748337
GAGTCGC6850.036.78748342
GGCAAAG21550.036.2172717
GAGAGAG6900.036.0136738
TGCGTGG31600.035.3314710
GCGTGGG29250.035.2983411
TGCCTAG6150.035.2840566
GGGGCAA22250.034.9205515
CGGTGGA7350.034.76230633
CGTGGGG29800.034.64686212
GCCTAGT6250.034.15951567
CACGTAA5950.034.11686716
TGTCATT40350.033.6608122
Produced by FastQC (version 0.11.5)