FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n02_Sorb_0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n02_Sorb_0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5467148
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC264000.4828843118935138No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT184950.33829338441176277No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT142140.2599893033808487No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT138540.2534045173095735No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA108410.19829351610748416No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT105240.192495246150278No Hit
GTCTTTGGGTTAAGACTACGACGGTATCTGATCATCTTCGATCCCCTAAC90730.16595490006855496No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC88490.16185769984642814No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT83500.15273045470874394No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT82810.1514683707117495No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC82340.1506086903079997No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC81820.14965755454214885No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG81770.14956609918004782No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC80220.14673098295491543No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT78640.14384099351252244No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA76100.13919506111778937No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG73500.13443938228853508No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA71570.13090920531143477No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT70320.12862282125890867No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA69780.12763510334821737No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT68590.12545846573021252No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC65590.11997114400414986No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCG64950.11880051536925652No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATA64700.1183432385587513No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTA64350.11770305102404399No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT64290.11759330458952272No Hit
TACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAG63780.11666045989609207No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT63690.11649584024431019No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA63120.11545324911635829No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA62700.11468502407470953No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACT62600.11450211335050743No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA61270.11206940071861965No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA60960.11150237747359318No Hit
GGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTA60780.11117313817002941No Hit
GTTGTAGACGGCCTTGGTAGGTCTCTTGTAGACCGTCGCTTGCTACAATT60750.1111182649527688No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC60700.11102680959066775No Hit
GTGTAACAACTCACCGGCCGAATGAACTAGCCCTGAAAATGGATGGCGCT59960.10967327023157229No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT58850.10764296119292911No Hit
ACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAG58710.10738688617904618No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGG57040.10433227708487132No Hit
TAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTG56260.10290557343609502No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTA55470.10146057871489851No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA54980.10056431616630827No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA54940.10049115187662745No Hit
AACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGAC54770.1001802036454839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA13400.048.3242727
TTTGCGA8400.047.5033158
TGGGGCA29550.044.53331414
ATGTCAT53950.043.9326021
TGTCATT54050.043.5866472
GGGCAAA30800.043.2941216
GGCAAAG31050.042.94553417
GAGTCGC7200.042.2903642
TGCGTGG46400.041.93836210
CGCTAGG7250.041.5159646
GGTGATC7300.041.2316151
GTGATCG7050.041.20440352
CGTGGGG43100.040.76440412
GCGTGGG43550.040.6646511
TGCCTAG6800.040.66162566
CATTTTG58900.040.5315
GAGAGAG7800.039.93466638
GGAGAGA7900.039.42916537
GATAAAT62100.039.4006843
GGGGCAA34000.038.80762515
Produced by FastQC (version 0.11.5)