FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n02_SC_9_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n02_SC_9_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7592888
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT239150.31496579430646154No Hit
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC219700.28934971778854107No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT209430.27582390257830747No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC183090.24113354496997716No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG177990.23441673313237335No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT175380.2309793058978349No Hit
GGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAA160710.21165859420025687No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA155760.20513933565199433No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT139260.18340847382445258No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT129290.17027776519290158No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC126920.1671564232213092No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG124340.16375850664463903No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTAT118830.15650171581616903No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA108040.14229104920288566No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT106220.13989406929221132No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT105920.13949896271352877No Hit
GATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG97970.12902863837844045No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA97880.1289101064048357No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC97570.12850182960686368No Hit
GATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTC95360.125591211143902No Hit
ATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATC94810.12486684908298397No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT93090.12260157136520385No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC92290.1215479538220503No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA92170.1213899111905773No Hit
GGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTA91790.12088944285757937No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA90870.11967778268295277No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC90310.1189402504027453No Hit
GTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACG86610.11406726926566019No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTT86390.11377752444129295No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT86290.11364582224839878No Hit
ACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAG85670.11282926865245477No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA84830.11172297023214356No Hit
GCCATAATCCAGCGGATGGTAGCTTCGCGGCAATGCCTGATCAGACAGCC84510.11130152321488214No Hit
TAATTGAACTTAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTG84500.11128835299559271No Hit
TAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTG83190.10956305426867878No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTA80230.10566466935901069No Hit
GGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGA77280.10177945466863202No Hit
CCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGTTCAGC77190.10166092269502724No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACT77120.10156873116000131No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT76470.10071266690618906No Hit
GTGTCTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCT76450.10068632646761022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA10600.048.8673815
CACGTAA11550.044.84798416
TGGGGCA24250.044.45281614
CGCTAGG12600.044.44365346
GAGAGAG13100.043.01450338
GCTAGGT13000.041.7300347
GGGCAAA26200.041.54533416
AACACGT12450.040.76232514
GTGATCG13250.040.41437552
GAGTCGC14050.040.1060542
TGCCTAG13300.039.21034666
GCCTAGT14000.038.24957367
TTTGCGA10050.037.6130078
GGAGAGA15150.037.4250837
GGGGCAA29200.037.1571215
CGGTGGA15600.036.79494533
GCGTGGG39700.036.4099311
AAACGCG6500.036.07628313
CGTGGGG40450.035.9078912
CTAGTCT14900.035.70429669
Produced by FastQC (version 0.11.5)