FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n02_SC_6_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n02_SC_6_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10180382
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC267990.2632415954529015No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT235220.23105223359987867No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT224830.2208463297349746No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT184450.18118180634086226No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC175760.17264578087541313No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC169260.16626095170102653No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA166580.1636284375183564No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT166240.16329446183846538No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT159010.15619256723372463No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT152640.1499354346428258No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA151480.14879598820555065No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC149960.1473029204601556No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG148530.14589825804179057No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA136050.13363938602696834No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA135690.13328576471884845No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT134680.13229366049328994No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC126940.12469080236871269No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT125090.12287358175754111No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA116400.11433755629209198No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC111300.10932792109372713No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT111180.10921004732435385No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC109740.10779556209187435No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT107650.1057425939419562No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT107410.10550684640320963No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA106640.10475048971639767No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG105750.10387625926021245No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTAT105270.10340476418271927No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG104930.10307078850282829No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA104590.1027368128229373No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA102970.10114551693639787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA11350.048.1049115
CGCTAGG24250.044.3080946
TGGGGCA28550.044.13191614
CACGTAA12500.043.95925516
GAGTCGC25250.043.80080842
GGGCAAA29800.042.75095416
TGCCTAG23750.041.70496466
GTGATCG24200.041.65177552
GCCTAGT23750.041.41002367
GCTAGGT25700.041.39966647
GAGAGAG26950.040.51839438
AACACGT13650.040.51173414
GGAGAGA27450.040.16285737
CGGTGGA27300.040.1283133
CTAGTCT25000.039.33952769
GGTGATC26450.038.50558551
AGTCGCT29150.037.58046743
TGCGTGG54100.037.52210210
TAGTCTC25800.037.3057570
GGCAAAG34800.037.01086817
Produced by FastQC (version 0.11.5)