FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n02_SC_3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n02_SC_3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3734215
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC100140.2681688119189709No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT89280.23908639432919637No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT84090.22518789089540908No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT78510.2102449912498343No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA74630.19985458791205113No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC66810.1789131048962098No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT60570.16220276550761004No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC59140.15837331273105593No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA56520.15135711253904768No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT55740.14926832011547272No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG54820.1468046162312561No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT52560.14075247408089786No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC48490.12985326233224387No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA47510.12722888210775224No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA46700.12505975151403975No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT46590.1247651782235356No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA45740.12248893006963979No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC45740.12248893006963979No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT45460.12173910714835649No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT44000.11782931620166487No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC43670.11694559633015239No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA43360.11611543523873157No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA41940.1123127618522233No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATA41870.11212530612190247No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA41580.1113487038105733No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT41020.10984905796800667No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT40350.10805483883493586No Hit
GTTGTAGACGGCCTTGGTAGGTCTCTTGTAGACCGTCGCTTGCTACAATT40020.10717111896342336No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC39830.10666231055255254No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTA39670.10623384031181922No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGG39300.10524300288012339No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG38970.10435928300861091No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG38810.10393081276787758No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACT37580.10063694779224014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGC7450.046.0392842
GAGAGAG7400.045.87739638
GTGATCG7200.045.20736752
GCTAGGT7600.044.20958347
CGCTAGG7450.044.16012646
GCCTAGT7400.043.5125867
TGGGGCA11400.043.28854814
CGGTGGA7550.043.11281233
CGGGTGA2550.042.5515257
TAGTCTC7500.042.46575570
TGCCTAG7550.041.7209666
ACACGTA2300.041.08662415
GGTGATC8000.040.68662651
TGCGTGG18900.040.55468810
GGAGAGA8300.040.48106437
GGGCAAA12400.040.3625816
ATATCTA15350.040.3623053
GCGGGTG2700.040.1891676
GCGTGGG17450.039.9131911
CTAGTCT8050.039.56437369
Produced by FastQC (version 0.11.5)