FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n02_SC_3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n02_SC_3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10272970
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT244880.23837312870571997No Hit
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC244790.23828552015629367No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT225220.21923552779770603No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG215220.20950124452811603No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT204250.19882273578137577No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC201360.19600952791646425No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT179900.17511975601992413No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC150970.14695847452100025No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC144660.14081614177788895No Hit
GTGTCTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCT141870.13810027674567335No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT139480.13577378304424134No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA139270.13556936309557996No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCG136260.13263934383143336No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG126610.12324576047627901No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA124230.12092900105811659No Hit
GGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAA123940.12064670684329849No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG123180.11990690131480965No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC122610.119352047168443No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT119520.11634415363813971No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT117780.11465038834923104No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT115790.11271326597858264No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA114660.11161329196911897No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT114040.11100976640640438No Hit
TAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTG111250.10829390137418876No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA110890.10794346717648354No Hit
TTGCTAGCCTGCTATGGTTCAGCGACGCCACAAGGACGCCTTATTCGTAT108960.10606475050545267No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG106440.10361171112151597No Hit
GTGTAACAACTCACCGGCCGAATGAACTAGCCCTGAAAATGGATGGCGCT105680.10287190559302714No Hit
GCATAAAATCAGGCCTTTGAAACGGAGCTTCCCCATCTCTTAGGATCGAC104240.10147016880220619No Hit
GATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTC103280.10053567760832553No Hit
GTCTAGGCTTCGTCACTGACCTCCACGCCTGCCTACTCGTCAGGGCATCA102980.10024364911023784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGCA42500.046.2811814
GGGCAAA43600.045.1944716
GGCAAAG46850.041.9846117
TGCGTGG70450.040.63752410
GGGTTAA17200.040.496127
GCGTGGG66300.040.48897611
CGTGGGG67050.039.98407412
CGCTAGG17700.036.97628846
GGGGCAA53700.036.69420615
TGTCATT92200.035.9160962
GAGAGAG18650.035.84342238
GCTAGGT17800.035.58881447
ATGTCAT93750.035.5886761
GTGATCG17450.035.29979352
TTTGCGT83500.034.8339968
CATTTTG95350.034.691845
GAGTCGC19150.034.5420442
GCCTAGT16800.034.37412667
TGCCTAG16900.033.9641366
TTTGCGA11200.033.751618
Produced by FastQC (version 0.11.5)