FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n02_SC_0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n02_SC_0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9253548
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT228660.24710521845242497No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT208010.22478945373169296No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT196140.2119619415169187No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG193240.20882800845686433No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC191920.20740152858125335No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC185650.20062574917210133No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA170470.18422123060257536No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT165450.178796284408964No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC140590.151930913418291No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC135560.14649516055895534No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA131410.1420103942833603No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA124560.13460782826219736No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC124100.1341107216388784No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT123450.13340828836679725No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG122110.13196019515973764No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA119960.12963676202900767No Hit
GTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACG118450.12800495550463453No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT116590.12599491567991003No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA111830.12085094279513113No Hit
GCCATAATCCAGCGGATGGTAGCTTCGCGGCAATGCCTGATCAGACAGCC110670.11959736957110936No Hit
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC110270.11916510294213635No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT110060.11893816296192551No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA109540.11837621634426061No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT108150.11687408980857936No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTA107400.11606358987925497No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT106370.11495050330964944No Hit
GTAAAGAAACTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCAC103670.11203270356408157No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT103210.11153559694076262No Hit
GATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTC102670.11095203699164903No Hit
GTATTTCACTGGCGCCGAAGCTCCCACTTATTCTACACCCTCTATGTCTC101990.11021718372239492No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA101700.10990379041638948No Hit
GCATAAAATCAGGCCTTTGAAACGGAGCTTCCCCATCTCTTAGGATCGAC99100.10709405732806487No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA98760.1067266306934378No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG98650.10660775737047021No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC97560.10542983080651874No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT97190.10502998417471872No Hit
GACTTAGAGGCGTTCAGCCATAATCCAGCGGATGGTAGCTTCGCGGCAAT97100.10493272418319978No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA94830.10247961106377793No Hit
GGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTA93620.10117200451113455No Hit
GTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGAT93540.10108555118533995No Hit
GGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTA92560.10002649794435604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA9000.049.38842415
AACACGT10150.044.4816214
AAACGCG6200.042.33762413
CACGTAA10800.041.4810916
TTTGCGA7750.038.388818
TGGGGCA20400.037.91569514
AACGCGT7200.035.971314
GGGCAAA21850.035.72048616
CGCTAGG7600.034.5385946
GAGTCGC8050.033.477442
GCTAGGT8100.032.83866547
GAGAGAG8250.032.6658338
GTGATCG7450.032.41527652
GGCAAAG24700.031.59889217
GGAGAGA8650.030.34604537
CGGTGGA9300.029.73138633
GCCTAGT8050.029.56509867
CAAACGC9250.029.1343912
TGCCTAG8000.028.87488466
TAGTCTC8000.028.87488470
Produced by FastQC (version 0.11.5)