FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14605947
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC201500.13795750456988512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG197330.13510250311054806No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT180160.12334701748541194No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT154890.10604584557235487No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT149780.10254727064256772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGG13000.043.59047346
GTGATCG12150.042.00333852
TGGGGCA31450.040.75818614
TGCGTGG47800.038.7855110
GCGTGGG44250.038.5832211
GGGCAAA33200.038.50629816
GCTAGGT14800.038.26766647
CTAGGTG14300.038.1352948
CGTGGGG45150.037.89145312
CGGTGGA15900.035.95076833
GAGAGAG16900.035.8528238
GGAGAGA17200.035.0304737
GGGGCAA36550.034.97543715
GGCAAAG38500.033.839717
TGCCTAG13900.033.81894366
GGTGATC15150.033.6911651
GCCTAGT14250.032.74651367
ATGTCAT66800.032.2301521
CTAGTCT14900.031.08068569
TAGTCTC13900.030.53852770