FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n01_Sorb_9_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n01_Sorb_9_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10906063
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT246130.22568180653275155No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT235520.21595327296385505No Hit
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC222410.20393243648051548No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT208330.19102218646637198No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC194190.17805692118228184No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA192870.17684658524345587No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT192400.17641563229554058No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC181260.16620113050878213No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC179690.16476156427851185No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA176480.16181824733636693No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT168810.15478546199485552No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG162680.14916473524864104No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT151050.13850094209065178No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA149430.13701552980209267No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA139220.12765376469950704No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA137050.12566404576977044No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC136640.1252881080918018No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATA136110.12480213987393984No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT135940.1246462632757577No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT135630.12436201771436678No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA134790.12359180393511388No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT127950.1173200631611976No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA126000.11553206688793197No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC123510.11324893318514664No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTA122470.11229533517273832No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT122030.11189188985979634No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC120330.11033312387797503No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC117580.10781159067208762No Hit
GTAAATACCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGC117390.10763737565058994No Hit
GTTGTAGACGGCCTTGGTAGGTCTCTTGTAGACCGTCGCTTGCTACAATT116890.10717891506770133No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG116590.10690383871796816No Hit
AATTAAAACATAGCATTGCGATGGTCAGAAAGTGATGTTGACGCAATGTG115250.10567516435582666No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA114800.1052625498312269No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT113610.10417141364395198No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTC113600.10416224443229423No Hit
AGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTC113020.10363043015614343No Hit
GAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAT111770.10248427869892188No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT110440.10126477354843814No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGG109410.10032034474768758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGG17550.044.27548246
GAGTCGC18000.043.94640742
GTGATCG17100.042.3670152
GCTAGGT18650.041.4733447
AAACGCG5500.041.3622613
ACACGTA8800.040.17077615
TGCCTAG17000.039.9398566
GCCTAGT17550.039.2864467
CTAGGTG19800.038.8877748
CGGTGGA21050.037.74411433
TGGGGCA25450.036.7193514
AGTCGCT21700.036.4532443
CACGTAA9550.036.28301216
GCGTGGG38150.035.96210511
CTAGTCT19500.035.71676369
CGTGGGG38250.035.68508512
TGCGTGG42000.035.4154910
AACACGT10250.034.82954414
GGAGAGA23500.034.70097737
GGGCAAA27100.034.4836716
Produced by FastQC (version 0.11.5)