FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n01_SC_9_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n01_SC_9_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7592888
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT262230.34536266042644115No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT224460.29561874217030465No Hit
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC214960.28310703384535635No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC195930.25804410653759147No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT184960.2435963759770986No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG178180.2346669672988723No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA170940.22513172853333277No Hit
GGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAA168790.22230013138610766No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG144900.19083647750368504No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT136630.17994470615133532No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC132970.17512440589140785No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT131480.17316204321728437No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTAT118000.15540858761514723No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT113920.15003513814506417No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA110360.14534654007803094No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT109460.1441612203419832No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA108580.14300224104451428No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA106740.14057892069526115No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC103530.13635128030335758No Hit
GATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG101170.13324310855105462No Hit
GATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTC98910.13026663899164587No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA96950.1276852760109197No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT96820.12751406316015723No Hit
TAATTGAACTTAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTG96240.12675019044137092No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC95750.12610484969618938No Hit
GCCATAATCCAGCGGATGGTAGCTTCGCGGCAATGCCTGATCAGACAGCC93130.12265425224236154No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC93050.12254889048804618No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA91470.12046799584031795No Hit
ACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAG88370.11638522786059798No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT87710.11551599338749631No Hit
TAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTG86880.11442286518647451No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACT86350.11372484356413529No Hit
ATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATC85710.11288194952961245No Hit
GTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACG82150.10819335146257919No Hit
GGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTA80900.10654707405140179No Hit
CAATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATA79490.10469007313159368No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT78560.10346524273767768No Hit
TTGCTAGCCTGCTATGGTTCAGCGACGCCACAAGGACGCCTTATTCGTAT77980.10270137001889136No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC77920.10262234870315484No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG77120.10156873116000131No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTA76310.10050194339755836No Hit
CTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAA76220.10038341142395357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA11350.050.57311615
CACGTAA12600.045.55594616
AAACGCG6700.044.40190513
TGGGGCA25450.042.63297714
AACACGT13700.042.40912214
CGCTAGG13400.041.79250346
GAGTCGC14200.041.1634142
GGGCAAA26300.040.9889416
GCTAGGT13350.040.6357147
GGAGAGA15700.038.56593737
GAGAGAG15250.038.55643538
GTGATCG13300.038.42011652
AACGCGT7800.038.14135414
GCCTAGT13400.038.133467
CTAGGTG14450.037.78454648
TGCCTAG13450.037.47120766
GGGGCAA29200.037.03798715
GCGTGGG40500.036.46824611
TGCGTGG42900.036.3860610
CGTGGGG40850.036.07011412
Produced by FastQC (version 0.11.5)