FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n01_SC_6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n01_SC_6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5017912
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC167120.3330468928111932No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT142490.28396273190920845No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT129100.25727832612449164No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT104300.20785537889066208No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA87880.1751326049560056No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT84270.16793837755624252No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC78280.15600114151065222No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT77320.1540879951661169No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT76660.15277270705424886No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC75470.15040120273133525No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC74310.14808948423168838No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA72510.14450233483568464No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC72320.1441236912883287No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC71250.141991330258482No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT69720.13894225327187884No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT67820.13515581779831928No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA64980.1294960931957356No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG64740.12901780660960177No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA63980.12750323242017794No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC61580.12272036655883961No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG59290.11815671538281261No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA57690.11496813814192038No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA57600.1147887806721202No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT57220.11403149357740829No Hit
TACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAG57160.11391192193087483No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT56950.11349342116800773No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA56900.11339377812922985No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA56600.11279591989656254No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA53940.10749491023357924No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACT52710.10504369147964333No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT51920.10346933146695278No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGG51830.1032899739971526No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT51110.1018551142387511No Hit
GTAAATACCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGC50860.10135689904486168No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG50330.10030068283381614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGCA24000.049.1457614
GGGCAAA25200.046.66659516
CGCTAGG7750.045.1639346
GAGTCGC8350.044.01454542
GGCAAAG26850.043.7988217
GTGATCG7650.043.46269252
GCTAGGT8000.043.31114247
TTTGCGA6950.043.3088
TGCGTGG37100.043.01752510
GCGTGGG34800.042.74291211
CTAGGTG8250.042.42291648
TGCCTAG7600.042.36709266
CGTGGGG35300.042.1374912
GGGGCAA28050.042.0498515
GGGTTAA8100.041.9122667
GCCTAGT7850.041.46366567
ATGTCAT45800.041.273721
TGTCATT47100.040.275552
GGAGAGA9550.039.94639637
GAGAGAG9600.039.7383438
Produced by FastQC (version 0.11.5)