FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n01_SC_3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n01_SC_3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5713807
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT178990.31325874325121583No Hit
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC176280.30851584591499154No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT161380.2824386612988503No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC143270.2507435060372183No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT142910.25011345325454637No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG131590.2303017935327532No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT131230.22967174075008132No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA123690.21647563524634278No Hit
GGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAA116220.2034020400059015No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG106780.1868806559269503No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC92320.16157353582296358No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT81400.14246193474858357No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA80730.14128933651416647No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT80610.14107931891994252No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT78430.13726399929154065No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC77470.135583858537749No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG77280.13525133068022774No Hit
TAATTGAACTTAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTG76020.1330461459408762No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC74720.13077095533678335No Hit
GATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTC74380.1301759054864821No Hit
GATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAG74330.13008839815555548No Hit
GCCATAATCCAGCGGATGGTAGCTTCGCGGCAATGCCTGATCAGACAGCC74220.12989588202751687No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTAT73510.12865327792835846No Hit
TAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTG71280.12475045096902992No Hit
ACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAG69460.12156518412329993No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACT68480.11985004043713761No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA68360.11964002284291367No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC67700.1184849260746819No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA67060.11736483223882081No Hit
TACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAG64180.11232440997744587No Hit
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT64010.11202688505229526No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA62960.11018923110283564No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCG61940.10840408155193201No Hit
GTGTCTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCT61790.10814155955915206No Hit
GTAAATACCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGC61470.10758151264122152No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC60050.1050963044429047No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA59440.1040287150055996No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT59300.10378369447900498No Hit
CAATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATA58920.10311863876396245No Hit
TTGCTAGCCTGCTATGGTTCAGCGACGCCACAAGGACGCCTTATTCGTAT58860.10301362996685047No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT58570.1025060874474759No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTA57390.10044091443760701No Hit
CTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAA57250.1001958939110124No Hit
GTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACG57200.10010838658008575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA5500.048.9998915
CGCTAGG12100.046.86186246
TGGGGCA23450.045.82079314
GCTAGGT12650.045.09752747
GGGCAAA24000.044.7707316
GCCTAGT12050.044.4385767
TGCCTAG11900.044.410566
GTGATCG12550.044.34136652
GAGAGAG13550.044.16848438
AAACGCG3400.043.23406213
GGAGAGA14050.042.84575737
CTAGGTG13000.042.80647348
GAGTCGC14000.042.50213642
GCGTGGG33300.042.2510511
TGCGTGG35500.042.0973910
TTTGCGA8150.042.084698
CTAGTCT12500.041.99880269
CGTGGGG33450.041.8523212
GGCAAAG26300.040.8554217
CGGTGGA14750.040.10230633
Produced by FastQC (version 0.11.5)