FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n01_SC_0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n01_SC_0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9253548
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT250370.27056648973993547No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT214030.23129506649773685No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC213270.23047375990268815No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT207630.22437880043416858No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC194760.2104706216469618No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG189340.2046134088243774No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT179320.19378512976860335No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA174320.18838179690644066No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA169360.18302169070717522No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC149690.16176497922742714No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC142850.15437321987198854No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG138430.14959667362183673No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT136000.14697065385082564No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC133290.14404204743953347No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA133210.14395559411373887No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA129990.1404758477505061No Hit
GCCATAATCCAGCGGATGGTAGCTTCGCGGCAATGCCTGATCAGACAGCC126330.13652060809540298No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT123430.13338667503534862No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT122560.13244649511733228No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTA119730.1293882087173482No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA119440.12907481541134277No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT118050.12757268887566153No Hit
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC117300.12676218894633712No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG116200.12557345571666134No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT114320.12354180256048816No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT114100.123304055914553No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA113360.12250436265095291No Hit
GTAAAGAAACTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCAC111840.12086174946085546No Hit
GTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACG109910.11877606297606065No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT108830.11760894307783351No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC107530.11620407653367121No Hit
CTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAA105530.11404274338880611No Hit
GATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTC103930.11231367687291405No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACG102700.11098445698882202No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA102600.11087639033157877No Hit
TAATTGAACTTAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTG101110.10926619713865428No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA100290.10838005054925959No Hit
GTATTTCACTGGCGCCGAAGCTCCCACTTATTCTACACCCTCTATGTCTC97490.10535418414644848No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT96900.10471659086871328No Hit
CTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGC96720.10452207088567543No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA95140.10281461770123201No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG94870.10252283772667521No Hit
GAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTTTATAGTTT94650.10228509108074005No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA94330.10193927777756165No Hit
GACTTAGAGGCGTTCAGCCATAATCCAGCGGATGGTAGCTTCGCGGCAAT94280.10188524444894001No Hit
ATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGGCGTG93380.10091264453375073No Hit
GCATAAAATCAGGCCTTTGAAACGGAGCTTCCCCATCTCTTAGGATCGAC93160.10067489788781556No Hit
GGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTA92870.10036150458181013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA5850.055.04268315
TTTGCGA8500.049.4106528
AACACGT7050.045.67371714
AAACGCG5750.045.0424613
CACGTAA7500.042.93329216
GAGAGAG7500.041.0657438
CGCTAGG7700.039.0921446
TGGGGCA23550.038.49253514
GGGCAAA23500.038.425516
GGAGAGA8100.038.02383437
AACGCGT6900.037.53619814
CTAGGTG7850.037.45138548
GAGTCGC8350.036.46821242
GCTAGGT8150.035.64336847
GTGATCG7600.035.4597352
GGCAAAG25550.035.34243417
CGGTGGA9250.034.0543933
GCCTAGT7550.033.84029867
TTGCACC6250.033.60105540
TGCCTAG7550.033.3767366
Produced by FastQC (version 0.11.5)