FastQCFastQC Report
Fri 1 Mar 2019
HFWYVAFXY_n01_SC_0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYVAFXY_n01_SC_0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7192085
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC246780.34312720163902405No Hit
ATGTCATTTTGCGTGGGGCAAAGAAATCACCGCGTTCTAGCATGGATTCT238380.33144769562651166No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT174400.24248879149787578No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC145240.20194422062586856No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT129500.18005905102623232No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT126740.17622149905069254No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT124360.1729123056804807No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC118170.16430562208316504No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA110180.15319618719745387No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGC109180.15180576981501193No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT107280.14916397678837223No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGG103170.14344936134653583No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT101590.14125250188227753No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC99830.1388053672891797No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC96740.13450897757743407No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA96080.1335913021050224No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG95670.1330212309782212No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA95660.13300732680439678No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT95330.13254848906819094No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC93890.13054628803747453No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG93810.13043505464687918No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA93160.1295312833482919No Hit
ATGTAAATACCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCA92800.1290307330906128No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA92330.12837723692086508No Hit
TACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAG89580.12455358911914974No Hit
TATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGGCCCGGT88230.1226765256528531No Hit
ATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGGCCCGG87990.12234282548106704No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA87300.12138343748718208No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCG86580.12038233697182388No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA85850.11936733228264126No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACT84410.11736513125192485No Hit
ATACAGGGCCCATTCGGGTCTTGTAATTGGAATGAGTACAATGTAAATAC84230.11711485612308531No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA82680.1149597091803003No Hit
GTGTCTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCT82480.1146816257038119No Hit
TAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTG80820.11237353284895826No Hit
GTAAATACCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGC80610.11208154519864547No Hit
GTACAATGTAAATACCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGC80070.1113307198121268No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA79020.10987078156056275No Hit
GATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTC78040.10850817252576965No Hit
GTCTTTGGGTTAAGACTACGACGGTATCTGATCATCTTCGATCCCCTAAC77670.10799371809426613No Hit
GGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAA77570.10785467635602193No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT76720.10667282158094628No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCATCTTCGATCCCCTAACT76610.10651987566887766No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG76530.1064086422782823No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC76250.10601932541119856No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA75800.10539363758909968No Hit
AGCCTAGACCGTAAGGTCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTG75200.1045593871596345No Hit
GTATAGGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCG73950.10282136543158207No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTA73870.10271013204098671No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT73500.1021956776094832No Hit
ACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAG73410.10207054004506343No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA73220.10180636074239946No Hit
GAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTA72410.10068012266262148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA13050.050.9565437
CGCTAGG8450.049.2917646
TGGGGCA38300.048.4331814
GCTAGGT8650.047.7447747
GTGATCG8200.046.95001252
GAGAGAG9000.046.66546638
GGGCAAA39950.046.5204216
TTTGCGA7650.046.2079628
CTAGGTG8900.046.01037248
GGCAAAG41400.045.0601717
TGCCTAG8100.044.9371266
GGAGAGA9450.044.81366337
GCCTAGT8200.044.38910367
ATGTCAT67550.043.8416021
TGTCATT68000.043.4942472
GGGGCAA43000.043.30211315
TGCGTGG59450.043.27054610
GCGTGGG55250.043.07581311
GAGTCGC9950.042.91615342
CGTGGGG55900.042.63754312
Produced by FastQC (version 0.11.5)