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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2018-12-20, 11:12 based on data in: /beegfs/mk5636/logs/html/HFWYLAFXY/merged


        General Statistics

        Showing 128/128 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HFWYLAFXY_n01_AP1-A09
        16.1%
        41%
        0.4
        HFWYLAFXY_n01_AP1-B07
        35.6%
        39%
        4.5
        HFWYLAFXY_n01_AP1-C04
        32.8%
        39%
        3.9
        HFWYLAFXY_n01_AP1-E04
        34.3%
        40%
        3.9
        HFWYLAFXY_n01_AP1-F01
        31.2%
        39%
        3.3
        HFWYLAFXY_n01_AP2-D01
        28.5%
        39%
        2.9
        HFWYLAFXY_n01_AP2-D10
        23.3%
        38%
        1.5
        HFWYLAFXY_n01_AP2-E01
        22.9%
        39%
        1.7
        HFWYLAFXY_n01_AP2-E06
        19.2%
        39%
        1.2
        HFWYLAFXY_n01_AP2-E07
        23.6%
        39%
        2.0
        HFWYLAFXY_n01_AP2-F12
        21.5%
        39%
        1.7
        HFWYLAFXY_n01_AP3-A11
        27.5%
        39%
        2.2
        HFWYLAFXY_n01_AP3-A12
        25.7%
        39%
        2.1
        HFWYLAFXY_n01_AP3-C12
        23.7%
        39%
        1.7
        HFWYLAFXY_n01_AP3-D01
        16.3%
        39%
        0.9
        HFWYLAFXY_n01_AP3-D05
        28.4%
        39%
        2.3
        HFWYLAFXY_n01_AP3-E06
        19.8%
        38%
        1.1
        HFWYLAFXY_n01_AP3-E09
        20.8%
        38%
        1.2
        HFWYLAFXY_n01_AP3-E10
        19.9%
        38%
        1.3
        HFWYLAFXY_n01_AP3-E11
        24.8%
        39%
        2.0
        HFWYLAFXY_n01_AP3-F02
        30.0%
        40%
        2.7
        HFWYLAFXY_n01_AP3-F03
        28.4%
        39%
        2.8
        HFWYLAFXY_n01_AP3-F05
        24.1%
        38%
        1.9
        HFWYLAFXY_n01_AP3-F07
        27.0%
        40%
        1.8
        HFWYLAFXY_n01_AP3-F09
        22.3%
        39%
        1.7
        HFWYLAFXY_n01_AP3-F11
        23.5%
        39%
        1.8
        HFWYLAFXY_n01_AP3-G02
        22.9%
        39%
        1.9
        HFWYLAFXY_n01_AP3-G05
        26.3%
        39%
        2.1
        HFWYLAFXY_n01_AP3-H02
        22.9%
        38%
        1.4
        HFWYLAFXY_n01_AP3-H03
        23.2%
        38%
        1.8
        HFWYLAFXY_n01_AP3-H05
        23.4%
        38%
        1.4
        HFWYLAFXY_n01_AP3-H07
        25.7%
        38%
        2.0
        HFWYLAFXY_n01_AP3-H09
        23.2%
        38%
        1.5
        HFWYLAFXY_n01_AP3-H11
        21.9%
        38%
        1.3
        HFWYLAFXY_n01_DGY1972
        11.9%
        38%
        0.4
        HFWYLAFXY_n01_DGY2071
        23.3%
        39%
        1.7
        HFWYLAFXY_n01_DGY2076
        25.0%
        38%
        1.1
        HFWYLAFXY_n01_DGY2084
        25.6%
        39%
        1.9
        HFWYLAFXY_n01_DGY2086
        28.0%
        39%
        2.0
        HFWYLAFXY_n01_DGY2089
        25.5%
        38%
        1.8
        HFWYLAFXY_n01_DGY2090
        29.6%
        39%
        2.5
        HFWYLAFXY_n01_DGY2135
        28.6%
        38%
        2.5
        HFWYLAFXY_n01_DGY2143
        23.6%
        38%
        1.5
        HFWYLAFXY_n01_DGY2144
        27.2%
        40%
        2.5
        HFWYLAFXY_n01_DGY2150
        27.1%
        39%
        2.1
        HFWYLAFXY_n01_DGY2151
        27.4%
        40%
        2.0
        HFWYLAFXY_n01_Gbc_c67
        30.4%
        40%
        1.8
        HFWYLAFXY_n01_Gbc_c70
        29.0%
        40%
        1.6
        HFWYLAFXY_n01_Gbc_c72
        6.6%
        41%
        0.1
        HFWYLAFXY_n01_Gbc_c73
        30.1%
        40%
        3.0
        HFWYLAFXY_n01_Gbc_c75
        25.6%
        39%
        2.4
        HFWYLAFXY_n01_Gbc_c76
        16.6%
        42%
        0.4
        HFWYLAFXY_n01_Gbc_c78
        8.4%
        41%
        0.1
        HFWYLAFXY_n01_Gbc_c79
        5.4%
        44%
        0.0
        HFWYLAFXY_n01_Gbc_c80
        12.2%
        42%
        0.3
        HFWYLAFXY_n01_Gbc_c81
        29.2%
        40%
        2.9
        HFWYLAFXY_n01_Gbc_c82
        12.8%
        41%
        0.2
        HFWYLAFXY_n01_Gbc_c84
        27.5%
        40%
        1.7
        HFWYLAFXY_n01_bc_anc1
        25.9%
        39%
        2.5
        HFWYLAFXY_n01_bc_anc2
        20.9%
        39%
        1.7
        HFWYLAFXY_n01_bc_anc3
        32.7%
        40%
        2.9
        HFWYLAFXY_n01_bc_anc4
        29.3%
        39%
        2.0
        HFWYLAFXY_n01_bc_anc5
        26.9%
        39%
        1.6
        HFWYLAFXY_n01_undetermined
        69.9%
        42%
        11.3
        HFWYLAFXY_n02_AP1-A09
        16.1%
        41%
        0.4
        HFWYLAFXY_n02_AP1-B07
        35.3%
        39%
        4.5
        HFWYLAFXY_n02_AP1-C04
        32.4%
        39%
        3.9
        HFWYLAFXY_n02_AP1-E04
        33.4%
        40%
        3.9
        HFWYLAFXY_n02_AP1-F01
        30.7%
        39%
        3.3
        HFWYLAFXY_n02_AP2-D01
        28.2%
        39%
        2.9
        HFWYLAFXY_n02_AP2-D10
        23.0%
        38%
        1.5
        HFWYLAFXY_n02_AP2-E01
        22.5%
        39%
        1.7
        HFWYLAFXY_n02_AP2-E06
        18.8%
        39%
        1.2
        HFWYLAFXY_n02_AP2-E07
        23.2%
        39%
        2.0
        HFWYLAFXY_n02_AP2-F12
        21.2%
        39%
        1.7
        HFWYLAFXY_n02_AP3-A11
        27.2%
        39%
        2.2
        HFWYLAFXY_n02_AP3-A12
        25.4%
        39%
        2.1
        HFWYLAFXY_n02_AP3-C12
        23.1%
        39%
        1.7
        HFWYLAFXY_n02_AP3-D01
        16.2%
        39%
        0.9
        HFWYLAFXY_n02_AP3-D05
        28.1%
        39%
        2.3
        HFWYLAFXY_n02_AP3-E06
        19.7%
        38%
        1.1
        HFWYLAFXY_n02_AP3-E09
        20.3%
        38%
        1.2
        HFWYLAFXY_n02_AP3-E10
        19.5%
        38%
        1.3
        HFWYLAFXY_n02_AP3-E11
        24.4%
        39%
        2.0
        HFWYLAFXY_n02_AP3-F02
        29.9%
        40%
        2.7
        HFWYLAFXY_n02_AP3-F03
        28.0%
        39%
        2.8
        HFWYLAFXY_n02_AP3-F05
        24.1%
        38%
        1.9
        HFWYLAFXY_n02_AP3-F07
        26.6%
        40%
        1.8
        HFWYLAFXY_n02_AP3-F09
        22.0%
        39%
        1.7
        HFWYLAFXY_n02_AP3-F11
        23.2%
        39%
        1.8
        HFWYLAFXY_n02_AP3-G02
        22.7%
        39%
        1.9
        HFWYLAFXY_n02_AP3-G05
        26.0%
        39%
        2.1
        HFWYLAFXY_n02_AP3-H02
        22.6%
        38%
        1.4
        HFWYLAFXY_n02_AP3-H03
        22.8%
        39%
        1.8
        HFWYLAFXY_n02_AP3-H05
        23.1%
        38%
        1.4
        HFWYLAFXY_n02_AP3-H07
        25.6%
        39%
        2.0
        HFWYLAFXY_n02_AP3-H09
        23.1%
        38%
        1.5
        HFWYLAFXY_n02_AP3-H11
        21.6%
        38%
        1.3
        HFWYLAFXY_n02_DGY1972
        12.0%
        38%
        0.4
        HFWYLAFXY_n02_DGY2071
        23.2%
        39%
        1.7
        HFWYLAFXY_n02_DGY2076
        24.9%
        39%
        1.1
        HFWYLAFXY_n02_DGY2084
        25.4%
        39%
        1.9
        HFWYLAFXY_n02_DGY2086
        27.8%
        39%
        2.0
        HFWYLAFXY_n02_DGY2089
        25.2%
        39%
        1.8
        HFWYLAFXY_n02_DGY2090
        29.4%
        39%
        2.5
        HFWYLAFXY_n02_DGY2135
        28.6%
        38%
        2.5
        HFWYLAFXY_n02_DGY2143
        23.2%
        39%
        1.5
        HFWYLAFXY_n02_DGY2144
        26.9%
        40%
        2.5
        HFWYLAFXY_n02_DGY2150
        26.8%
        39%
        2.1
        HFWYLAFXY_n02_DGY2151
        26.9%
        40%
        2.0
        HFWYLAFXY_n02_Gbc_c67
        30.0%
        40%
        1.8
        HFWYLAFXY_n02_Gbc_c70
        28.8%
        40%
        1.6
        HFWYLAFXY_n02_Gbc_c72
        6.6%
        41%
        0.1
        HFWYLAFXY_n02_Gbc_c73
        29.8%
        40%
        3.0
        HFWYLAFXY_n02_Gbc_c75
        25.3%
        40%
        2.4
        HFWYLAFXY_n02_Gbc_c76
        16.4%
        42%
        0.4
        HFWYLAFXY_n02_Gbc_c78
        8.5%
        41%
        0.1
        HFWYLAFXY_n02_Gbc_c79
        5.2%
        44%
        0.0
        HFWYLAFXY_n02_Gbc_c80
        12.2%
        42%
        0.3
        HFWYLAFXY_n02_Gbc_c81
        29.1%
        40%
        2.9
        HFWYLAFXY_n02_Gbc_c82
        12.6%
        41%
        0.2
        HFWYLAFXY_n02_Gbc_c84
        27.3%
        40%
        1.7
        HFWYLAFXY_n02_bc_anc1
        25.4%
        39%
        2.5
        HFWYLAFXY_n02_bc_anc2
        20.4%
        39%
        1.7
        HFWYLAFXY_n02_bc_anc3
        32.4%
        40%
        2.9
        HFWYLAFXY_n02_bc_anc4
        29.0%
        39%
        2.0
        HFWYLAFXY_n02_bc_anc5
        26.5%
        39%
        1.6
        HFWYLAFXY_n02_undetermined
        68.1%
        42%
        11.3

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 64/64 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        11,296,202
        9.0
        bc_anc1
        2,450,948
        1.9
        Gbc_c73
        3,013,525
        2.4
        Gbc_c84
        1,741,187
        1.4
        AP2-E01
        1,705,336
        1.4
        AP3-D05
        2,344,559
        1.9
        AP3-F07
        1,791,427
        1.4
        AP3-H07
        2,009,028
        1.6
        DGY2089
        1,766,279
        1.4
        bc_anc2
        1,656,542
        1.3
        Gbc_c75
        2,448,466
        1.9
        AP1-A09
        441,314
        0.3
        AP2-E06
        1,222,243
        1.0
        AP3-E06
        1,120,640
        0.9
        AP3-F09
        1,723,916
        1.4
        AP3-H09
        1,495,596
        1.2
        DGY2090
        2,547,712
        2.0
        bc_anc3
        2,885,921
        2.3
        Gbc_c76
        393,985
        0.3
        AP1-B07
        4,525,708
        3.6
        AP2-E07
        1,964,781
        1.6
        AP3-E09
        1,178,467
        0.9
        AP3-F11
        1,811,153
        1.4
        AP3-H11
        1,258,454
        1.0
        DGY2135
        2,455,034
        1.9
        bc_anc4
        2,032,006
        1.6
        Gbc_c78
        74,568
        0.1
        AP1-C04
        3,862,820
        3.1
        AP2-F12
        1,655,126
        1.3
        AP3-E10
        1,250,800
        1.0
        AP3-G02
        1,856,305
        1.5
        DGY1972
        412,933
        0.3
        DGY2143
        1,520,746
        1.2
        bc_anc5
        1,647,043
        1.3
        Gbc_c79
        40,428
        0.0
        AP1-E04
        3,905,620
        3.1
        AP3-A11
        2,181,711
        1.7
        AP3-E11
        2,024,720
        1.6
        AP3-G05
        2,084,376
        1.7
        DGY2071
        1,654,504
        1.3
        DGY2144
        2,533,491
        2.0
        Gbc_c67
        1,783,796
        1.4
        Gbc_c80
        253,408
        0.2
        AP1-F01
        3,262,547
        2.6
        AP3-A12
        2,065,391
        1.6
        AP3-F02
        2,717,950
        2.2
        AP3-H02
        1,396,075
        1.1
        DGY2076
        1,139,505
        0.9
        DGY2150
        2,109,464
        1.7
        Gbc_c70
        1,603,255
        1.3
        Gbc_c81
        2,889,341
        2.3
        AP2-D01
        2,857,494
        2.3
        AP3-C12
        1,662,410
        1.3
        AP3-F03
        2,767,976
        2.2
        AP3-H03
        1,762,769
        1.4
        DGY2084
        1,928,994
        1.5
        DGY2151
        2,046,850
        1.6
        Gbc_c72
        66,468
        0.1
        Gbc_c82
        229,168
        0.2
        AP2-D10
        1,495,950
        1.2
        AP3-D01
        858,010
        0.7
        AP3-F05
        1,910,777
        1.5
        AP3-H05
        1,407,497
        1.1
        DGY2086
        1,974,859
        1.6

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        135,743,176
        126,175,574
        8.9
        4.9

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        All samples have sequences of a single length (76bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        128 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).